Abstract:  Excessive consumption of added sugars is a major contributor to diabetes and other non-communicable diseases, driving the global demand for low-sugar or sugar-free food products. Sucralose, a non-nutritive sweetener with high stability and sugar-like taste, is widely used in food, beverages, pharmaceuticals, and dietary supplements. It has been recognized as “Generally Recognized as Safe” (GRAS) by the U.S. Food and Drug Administration. However, its use must comply with national and international food safety regulations to ensure that acceptable daily intake (ADI) and maximum permitted levels are not exceeded. This study aimed to develop and validate a simple and reliable High-Performance Liquid Chromatography (HPLC) method for the quantification of sucralose in food and beverage samples available in the Sri Lankan market. Separation was achieved on a Hi-plex-H column (300 × 7.7 mm, 8 µm particle size) at 35°C using ultra-pure water as the mobile phase, monitored using a...